Chevron Left
Volver a Basics of Extracellular Vesicles

Opiniones y comentarios de aprendices correspondientes a Basics of Extracellular Vesicles por parte de Universidad de California en Irvine

328 calificaciones

Acerca del Curso

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Principales reseñas


10 de ene. de 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.


16 de may. de 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

Filtrar por:

1 - 25 de 89 revisiones para Basics of Extracellular Vesicles

por Shukhman B

12 de nov. de 2016

por Aedrian A

22 de ene. de 2021

por Margo M

29 de jun. de 2021

por Denise S

16 de mar. de 2017

por Minos V

31 de ene. de 2018

por Madhura K

5 de ago. de 2020

por Florencia I

30 de jun. de 2019

por Sandra M D A

8 de mar. de 2019

por Morgane G

16 de ago. de 2020

por Cahyani G A

5 de jun. de 2022

por Manasa V

29 de jun. de 2021

por Judith D M

1 de ene. de 2018

por Sai D K

15 de abr. de 2022

por Priyanka B

10 de abr. de 2020

por Samantha S

29 de ago. de 2016

por Momchil N

28 de mar. de 2020

por Santharam K S

9 de jun. de 2020

por Hasanul B

14 de oct. de 2016


8 de nov. de 2021

por Esteban O

15 de may. de 2019

por Azalea K

27 de jul. de 2018

por Nima K T

20 de feb. de 2021

por Jai M

2 de ene. de 2017

por Ashley R

30 de jun. de 2017

por Thais S M L

26 de may. de 2020