7.12 Molecular mechanism involved in neurotransmetter release VIII

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Del curso dictado por Peking University

Advanced Neurobiology I

258 calificaciones

Peking University

Advanced Neurobiology I

258 calificaciones

Hello everyone! Welcome to advanced neurobiology! Neuroscience is a wonderful branch of science on how our brain perceives the external world, how our brain thinks, how our brain responds to the outside of the world, and how during disease or aging the neuronal connections deteriorate. We’re trying to understand the molecular, cellular nature and the circuitry arrangement of how nervous system works. Through this course, you'll have a comprehensive understanding of basic neuroanatomy, electral signal transduction, movement and several diseases in the nervous system. This advanced neurobiology course is composed of 2 parts (Advanced neurobiology I and Advanced neurobiology II, and the latter will be online later). They are related to each other on the content but separate on scoring and certification, so you can choose either or both. It’s recommended that you take them sequentially and it’s great if you’ve already acquired a basic understanding of biology. Thank you for joining us!

De la lección

Neurotransmitter release

Let's learn more about the neurotransmitter release.

7.1 How does Ca2+ control neurotransmitter release I8:23

7.2 How does Ca2+ control neurotransmitter release II11:46

7.3 How does Ca2+ control neurotransmitter release III7:27

7.4 How does Ca2+ control neurotransmitter release IV10:18

7.5 Molecular mechanism involved in neurotransmetter release I7:56

7.6 Molecular mechanism involved in neurotransmetter release II7:11

7.7 Molecular mechanism involved in neurotransmetter release III5:27

7.8 Molecular mechanism involved in neurotransmetter release IV11:00

7.9 Molecular mechanism involved in neurotransmetter release V14:23

7.10 Molecular mechanism involved in neurotransmetter release VI12:35

7.11 Molecular mechanism involved in neurotransmetter release VII17:53

7.12 Molecular mechanism involved in neurotransmetter release VIII12:40

7.13 Molecular mechanism involved in neurotransmetter release IX7:59

7.14 Molecular mechanism involved in neurotransmetter release X13:25

Conoce a los instructores

Yan Zhang
Professor
School of Life Sciences, Peking University
Yulong Li
Research Fellow
School of Life Sciences, Peking University

Okay, so they do that experiment.

Again, in number one, it's the piece without an SF, okay?

That is, you just add this piece, but you don't add an SF, okay?

And then you go through this process, okay?

Y of 10 is in this page [INAUDIBLE].

You see some components here.

Okay, why now would there be some components there?

What are they?

Do you care?

Why there would be no components, what do you think?

They don't have a staff, why would there be some components there?

Okay because what you have is even though there's no SF, you still have the beads.

You still have this antibody there, okay?

You might bind to something right?

Hydrophobic interaction, non-specific interactions.

And indeed, this is likely due to the bee's or

the antibody coding how the bees can interact with some components.

Okay, that's the reason they need to do this control.

Because the purpose of this study is to identify the substrate for NSF, right?

It's not to study the hollow piece, or

the antibody itself can interact with some component.

This is not the purpose of the study, right?

And then, they wash, dilute, with magnesium ATP chrome s again.

You can see that there's some.

And this component is similar like number one, okay?

And this is again because you are using ATP kinase and

then you are this similarly like the piece.

This is probably also nonspecific.

And then this is the one that is go to mine.

So they added a magnesium ATP and inculbation and low and

behold YFI is aha!

Now you've got something interesting.

First, you've still got something here.

Okay, I don't care about what's on the right because they're

they're just similar like number one and number two, especially number two.

We renounce specific binding.

But in comparison between two and three.

You see there's additional components A, B, C, D, E.

Okay some of these things are a little bit weak so they label them.

There's also F here, okay?

So they can see in a much more convincing way here, okay?

So the in comparison with number 2 they

got more components here, okay?

And this will be the specific ones because it doesn't exist in

the ATP'S solution components, right, okay?

And this is a goldmine, okay?

ABCDEF.

Okay, now what are you going to do?

You're again, what if they already have these pure components?

They're just sequence in this protein.

They are pure, right?

We have already done this three times.

And that protein's experiment we identified a substrate,

we sequenced it adamine degradation.

We sequenced it from end terminal.

We can identify which amino acid in the sequence right,

and then we know which gene because of the central dogma.

The gene determine RNA, and then we can translate it into the proteins right.

You already count this a, b, c, d, e, f.

You just cut them out and individually sequence them.

That will be a 100,000 times easier than to try it,

because you don't know what to try.

Okay, so they sequence these components, okay?

So they got A is syntax in B, and C is the syntax in A.

Actually they are similar isoforms, okay?

And then E is a and

then F is one or two.

Okay, bingo.

If I am the author, I would be very excited to see those things.

Especially F, are you excited to see this?

F is labeling here.

It's 1 over [INAUDIBLE] bribing.

Two, I am very excited to see that.

Are you excited to see this?

The [INAUDIBLE] so if we fast backwards to this

paper that we discussed Saturday, right?

1992, October 29.

The group identify using this toxic,

this tetanus and bot-B toxin.

They said this toxin blocks transmitter release by

proteolytic cleavage of synaptobrevin.

Okay, they identified.

This synaptobrevin.

This synaptobrevin if you still remember.

This is synaptobrevin is the key component that have been clear and

after there's no transmitter release, right?

In 1992 October, and James Rothman in 1993

after about half a year in the same journal Nature,

using a totally different approach.

They're trying to identify an ATPA that he started for many years.

He wanted to understand these ETPAs, how you regulate vesicular fusion, okay?

And then he identify this ETPA's specific target.

At this one.

It's also a.

They are also using a extra.

And previously some of the students

using [INAUDIBLE] writing to do affinity purification

to identify the binding partners, right?

Probably the [INAUDIBLE] group just identified a [INAUDIBLE] in are happy

in doing the experiments which they are proposing to do.

So they are trying to prove that.

And then the [INAUDIBLE] after half a year.

Another group identified the contacts with the ATPA's packet.

And there is additional, at least, two proteins,

[INAUDIBLE] 25 and syntaxin will be in the contacts.

And these whole contacts will be the packet for ATPase.

So they might be happy or be crying, right?

So they will be, happy is great.

It's not working alone.

As we predicted, it has some additional component, right?

So this really makes sense, right?

So for different approaches, why is coming from a toxic people identify the.

And the other one who was trying to identify fusion, NSF especially,

and then they also come up with the along with the other components.

So in the early 1990's, the whole field are very excited,

because from all the different approaches, people come to the cent components

that might be essential for regulating transmitter release.

Indeed, we don't have time to discuss, but Randy Schekman,

who also get a Nobel Prize, through east, through the east genetics.

Also coming up with the mutants that is homolock

to the synatopribing, syanpt25, and the syntexted.

Okay so in the early 1990s, so this is an east paper.

Identify the complementation groups using yeast genetics important for

membrane fusion.

They also identify the whole mutant.

So, in the early 1990s people

are excited because of a converging evidence [INAUDIBLE] identify.

[INAUDIBLE] actually located near the plasma membrane okay?

Syntaxing, snap 25 are in the plasma membrane so

why is it in the basal membrane and the other is in the plasma membrane.

And they will follow context.

Does that make sense?

If you want to cause vesicle to fuse you will sort of predict

something will go on the vesicle membrane, something will go on the plasma membrane.

And somehow they work together to cause membrane fusion, right?

So indeed, James Rothman after identify,

the snap syntaxis and identify the synap worry,

they use just this so called snare component.

This is three proteins.

They pool in the liver zone, they pool in the liver zone and then they demonstrate.

In a liposome if you put them in the right amount,

then they are sufficient to drive liposome fusion.

So for example in this case this indicates that there's a liposome fusion, okay?

So just putting this protein in different liposome itself

It's sufficient to cause the membrane fusion okay?

So all this evidence and

if you are cleaning

using a toxin then there's no fusion.

Okay so all this evidence in vitro,

in vivo evidence all together make the feel very excited.

They identify the key components to cause membrane fusion and

that is how snares was identified.

And indeed from a structure people found actually this is similar,

it formed this helical structure.

And for virus to cause an infusion it turns out the virus using one protein,

one of its three protein, the virus is interesting in a way.

The virus will express a protein itself already has several helical domain.

But the other side, once they reach the target it will insert into the target's

membrane, and it will also undergo these similar structure arrangements.

So now people get some basic ideas.

There's key components there that might be important for Vesicle infusion.

And then it turns out that the are not calcium sensitive yet, okay?

And that's how it works, from and Alice identify

a calcium binding, but also enrich in a synaptic vesicle

a protein that is important for vesicle infusion.

And that's not can physically interact with the so-called SNARE complex, okay?

So therefore, all this is putting together, how

different molecules located at different places associate with each other.

And binds to calcium and then trigger transmitter reduce.

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