Acerca de este Curso
4.9
220 calificaciones
42 revisiones

100 % en línea

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Fechas límite flexibles

Restablece las fechas límite en función de tus horarios.

Aprox. 18 horas para completar

Sugerido: 14.5 hours of videos...

Inglés (English)

Subtítulos: Inglés (English)

100 % en línea

Comienza de inmediato y aprende a tu propio ritmo.

Fechas límite flexibles

Restablece las fechas límite en función de tus horarios.

Aprox. 18 horas para completar

Sugerido: 14.5 hours of videos...

Inglés (English)

Subtítulos: Inglés (English)

Programa - Qué aprenderás en este curso

Semana
1
1 horas para completar

Welcome

Welcome to the course!...
3 videos (Total 19 minutos), 3 readings
3 videos
Description of Parts7m
Introduction: Why Electrons?8m
3 lecturas
Welcome from Professor Jensen10m
About the Instructor10m
Concept Check Questions: Why Electrons?10m
Semana
2
3 horas para completar

Currents, Coils, Knobs and Names: Basic anatomy of the electron microscope

...
9 videos (Total 94 minutos), 8 readings
9 videos
Electron Guns18m
EM Lenses
The Column26m
Sample Chamber14m
Energy Filters7m
Detectors11m
Vacuum Systems9m
Summary and Safety5m
8 lecturas
Concept Check Questions: Electron Guns10m
Concept Check Questions: Electron Lenses10m
Concept Check Questions: The Column10m
Concept Check Questions: The Sample Chamber10m
Concept Check Questions: Energy Filters10m
Concept Check Questions: Electron Detectors10m
Concept Check Questions: Vacuum Systems10m
Concept Check Questions: Summary and Safety10m
Semana
3
3 horas para completar

Fourier Transforms and Reciprocal Space for Beginners

...
7 videos (Total 134 minutos), 6 readings
7 videos
1-D Sine Waves and their Sums32m
1-D Reciprocal Space20m
2-D Waves and Images19m
2-D Transforms and Filters32m
3-D Waves and Transforms13m
Convolution and Cross-Correlation15m
6 lecturas
Concept Check Questions: 1-D Sine Waves and Their Sums10m
Concept Check Questions: 1-D Reciprocal Space10m
Concept Check Questions: 2-D Waves and Images10m
Concept Check Questions: 2-D Transforms and Filters10m
Concept Check Questions: 3-D Waves and Transforms10m
Concept Check Questions: Convolution and Cross-correlation10m
Semana
4
4 horas para completar

Image Formation

...
7 videos (Total 164 minutos), 6 readings
7 videos
Amplitude and Phase Contrast30m
Wave Propagation and Phase Shifts30m
The Contrast Transfer Function33m
Defocus and its Effects21m
Envelopes16m
CTF Correction31m
6 lecturas
Concept Check Questions: Amplitude and Phase Contrast10m
Concept Check Questions: Wave Propagation and Phase Shifts10m
Concept Check Questions: The Contrast Transfer Function10m
Concept Check Questions: Defocus and its Effects10m
Concept Check Questions: Envelopes10m
Concept Check Questions: CTF Correction10m
Semana
5
2 horas para completar

Fundamental Challenges in Biological EM

...
6 videos (Total 96 minutos), 5 readings
6 videos
Sample Prep - Room Temperature Methods20m
Sample Prep Methods Involving Freezing27m
Sample Prep Grids11m
3-D Reconstruction11m
Dose Limitations22m
5 lecturas
Concept Check Questions: Sample Prep - Room Temperature Methods10m
Concept Check Questions: Sample Prep - Methods Involving Freezing10m
Concept Check Questions: Sample Prep - Grids10m
Concept Check Questions: 3-D Reconstruction10m
Concept Check Questions: Dose Limitations10m
Semana
6
4 horas para completar

Single Particle Analysis

...
5 videos (Total 159 minutos), 6 readings
5 videos
Data collection (imaging)21m
Reconstruction basics31m
Reconstruction: additional topics44m
Interpretation and limitations38m
6 lecturas
Concept check questions: Introduction to single particle analysis10m
Concept Check Questions: Special sample prep issues10m
Concept Check Questions: Data collection (imaging)10m
Concept Check Questions: Reconstruction basics10m
Concept Check Questions: Reconstruction - additional topics10m
Concept Check Questions: Interpretation and limitations10m
Semana
7
3 horas para completar

Tomography

...
5 videos (Total 131 minutos), 5 readings
5 videos
Tomography Data Collection and Reconstruction40m
Tomography Identifying Objects of Interest24m
Tomography Limitations29m
Tomography Parameters and Handedness12m
5 lecturas
Concept Check Questions: Introduction: Tomography10m
Concept Check Questions: Tomography Data Collection and Reconstruction10m
Concept Check Questions: Identifying Objects in Tomograms10m
Concept Check Questions: Tomography Limitations10m
Concept Check Questions: Tomography Parameters and Handedness10m
Semana
8
2 horas para completar

2-D Crystallography

...
5 videos (Total 55 minutos), 4 readings
5 videos
FT of a 2-D Crystal24m
2-D Crystallography Data Collection and Reconstruction17m
Helical Tubes3m
Farewell2m
4 lecturas
Concept Check Questions: Introduction: 2-D Crystallography and Sample Prep10m
Concept Check Questions: Fourier Transform of a 2-D Crystal10m
Concept Check Questions: Data Collection and Reconstruction10m
Concept Check Questions: Helical Tubes10m
4.9
42 revisionesChevron Right

Principales revisiones

por LRFeb 19th 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

por ALJul 20th 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

Instructores

Avatar

Grant J. Jensen

Professor of Biophysics and Biology; Investigator, Howard Hughes Medical Institute
Biology and Biological Engineering

Acerca de Instituto de Tecnología de California (Caltech)

Caltech is a world-renowned science and engineering research and education institution, where extraordinary faculty and students seek answers to complex questions, discover new knowledge, lead innovation, and transform our future. Caltech's mission is to expand human knowledge and benefit society through research integrated with education. We investigate the most challenging, fundamental problems in science and technology in a singularly collegial, interdisciplinary atmosphere, while educating outstanding students to become creative members of society....

Preguntas Frecuentes

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