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The course will cover the topics related to antimicrobial resistance with basic definitions and overview on antimicrobials their use and the emergence and spread of resistance. The course will guide you through the concepts and the importance of resistance spread and dissemination and how that happens. It will show you how bacteria become resistant and which mechanisms they might use for this. And as part of the course you will also receive some training in methods for antimicrobial susceptibility testing (AST) and detection of specific resistance in the microbiological laboratories with the basic methods available and with focus on the obtention of good quality results which can be interpreted and used for different purposes. Additionally, it will show you how to use genomic analysis tools to analyze whole genome sequencing data to detect resistance genes (and or other genes of interest) in a simple and easy way using online tools freely available. In the new new version an additional module including detection of specific resistance mechanisms was added. After this course you should be able to: 1. Describe the most important families of antimicrobials and mode of action 2. Understand the basic concepts of antimicrobial resistance from several perspectives (clinical, research and microbiological) 3. Enumerate and describe how bacteria can become resistant and the mechanisms that may be involved in that process 4. Describe how antimicrobial resistance emerges and spreads around the world including concepts of antimicrobial resistance transfer, selection and dissemination 5. Enumerate the methods used for antimicrobial susceptibility testing (AST) 6. Compare dilution and diffusion methods and know the basic techniques of agar disk diffusion, broth dilution and agar dilution methods 7. Have detailed theoretical knowledge on how to perform the main methods in a laboratory 8. Know the basic concepts about analysis and interpretation of results of AST, including different breakpoints, cut-off setting and their applications. 9. Understand the importance and related concepts related to quality management and quality assurance method standardization, applied to AST 10. Relate the information obtained in this course with real cases of resistant bacteria spreading in patients, the community, animals or the environment 11. Relate the phenotypical results with results from genotyping using molecular techniques for detection of resistance mechanisms 12. Understand the concept and be able to apply genomic analysis tools used to detect resistance genes and other relevant genes from Whole Genome Sequencing (WGS) data (with demonstration of selected online tools) Disclaimer: Please note that the guidelines and methods referred or links included in these materials are updated when the videos lectures are produced and before the course is released, however these might become outdated with time.
Globe

Cursos 100 % en línea

Comienza de inmediato y aprende a tu propio ritmo.
Intermediate Level

Nivel intermedio

Clock

Approx. 7 hours to complete

Sugerido: 5 weeks of study, 1-2 hours/week
Comment Dots

English

Subtítulos: English

Habilidades que obtendrás

MicrobiologyGenomeDrug ResistanceGenomics
Globe

Cursos 100 % en línea

Comienza de inmediato y aprende a tu propio ritmo.
Intermediate Level

Nivel intermedio

Clock

Approx. 7 hours to complete

Sugerido: 5 weeks of study, 1-2 hours/week
Comment Dots

English

Subtítulos: English

Syllabus - What you will learn from this course

1

Section
Clock
20 minutes to complete

Welcome to the course

This module is basically a welcome to the course were you can find information on the Course structure, the contents you will be learning in the next modules, the grading, and introduction to the instructors and to the teaching material....
Reading
1 video (Total 10 min), 1 reading
Video1 videos
Reading1 readings
NOTE: This version has been added an additional module on detection of Specific resistances10m
Clock
1 hour to complete

Module A- Antimicrobial and antimicrobial action

Antimicrobials and antimicrobial action ( includes two lectures and one quiz). Here you will learn a lot about antibiotics/ antimicrobials and you will know more about:what they are, where do they come from, what are the major groups and how can we classify them, how do they have an effect, how do they act on the bacterial cells...
Reading
2 videos (Total 41 min), 1 quiz
Video2 videos
Lecture 2- Antimicrobials and antimicrobial action16m
Quiz1 practice exercises
Quizz Lectures 1-2 (antimicrobials)10m

2

Section
Clock
1 hour to complete

Module B - Antimicrobials and resistance

This module containd information about antimicrobials and the development of resistance in bacteria including causes for resistance and their mechanisms, as well as an insight in how resistance disseminates and how it can be selected....
Reading
4 videos (Total 54 min), 1 quiz
Video4 videos
Lecture 4- Antimicrobials and resistance- causes and mechanisms14m
Lecture 5- Antimicrobial resistance- emergence and dissemination12m
Lecture 6- Antimicrobials and resistance - selection8m
Quiz1 practice exercises
Q2- Quizz Lectures 3-6 (antimicrobial resistance)10m

3

Section
Clock
2 hours to complete

Module C- Antimicrobial susceptibility testing

This module deals with antimicrobial susceptibility testing in the laboratory: its importance and use, descriptions of the methods and their applications and detailed descriptions of the procedures applied for the main methods....
Reading
5 videos (Total 108 min), 1 quiz
Video5 videos
Lecture 8- Antimicrobial susceptibility testing- methods17m
Lecture 9- Antimicrobial susceptibility testing- disk diffusion18m
Lecture 10- Antimicrobial susceptibility testing- agar dilution34m
Lecture 11- Antimicrobial susceptibility testing- broth dilution19m
Quiz1 practice exercises
Q3- Quizz Lectures 7-11 (AST)12m

4

Section
Clock
1 hour to complete

Module D- Interpretation

This module gives practical insight on how to interpret antimicrobial susceptibility results....
Reading
1 video (Total 29 min), 1 quiz
Quiz1 practice exercises
Q4- Quizz Lecture 12 (interpretation AST)8m
Clock
1 hour to complete

Module E- Quality assurance

This module is dedicated to Quality management and Quality assurance (QA/ Quality control (QC) procedures and use of QC strains to assure the qulaity of results of antimicrobial susceptibility testing: importance, elements and principles....
Reading
2 videos (Total 33 min), 1 quiz
Video2 videos
Lecture 14- Quality assurance when doing antimicrobial susceptibility testing22m
Quiz1 practice exercises
Q5- Quizz Lectures 13-14 (Quality assurance)6m

5

Section
Clock
1 hour to complete

Module F- Alternatives to AST- Genome analysis tools

As alternative to the phenotypical methods, the researcher might decide to use genotypically based methods to identify genes of interest. in the lectures of this module we present the Resfinder tool which may be used to find resistance mechanisms ( genes and in the future versions also specific point mutations)from Whole genome sequences of bacteria of interest. The second Lecture is a demonstration of the My DB Finder which can be used to identify genes of interest defined by the user from Whole genome sequence data....
Reading
2 videos (Total 23 min), 1 quiz
Video2 videos
Lecture 16- My Database Finder (MyDbFinder) tool principles and applications10m
Quiz1 practice exercises
Q6- Quizz Lectures 15-16 (WGS tools)20m
Clock
1 hour to complete

Module G- specific resistances

...
Reading
4 videos (Total 48 min), 1 quiz
Video4 videos
Beta-lactam resistance in staphylococcus aureus: MRSA13m
Colistin resistance in Enterobacteriaceae12m
Closing remarks to course completion3m
Quiz1 practice exercises
Quiz 730m
4.6
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Briefcase

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Top Reviews

By DJNov 15th 2016

It was really a good lectures and please I want to know about my certificate in have already purchased this course and submitted all\n\nThe documents let me know when will I get my certificate .

By AASep 24th 2017

This i very good course. i recommended to all microbiology and molecular biology students. All three teachers are very good. specially last week lecture about molecular detection of MRSA. CRE.

Instructors

Avatar

Lina Cavaco

Ext Researcher

Rene S. Hendriksen

PhD Senior Scientist

About Technical University of Denmark (DTU)

DTU focuses on research in technical and natural sciences that contributes to the development of society. As an industrially orientated university, our goal is to supply high-level international research based on combining theory with constructing models and empirical methods....

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