Acerca de este Curso
4.6
142 calificaciones
22 revisiones
Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging. A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content....
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Cursos 100 % en línea

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Fechas límite flexibles

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Sugerido: 6-8 hours/week

Aprox. 19 horas para completar
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English

Subtítulos: English
Globe

Cursos 100 % en línea

Comienza de inmediato y aprende a tu propio ritmo.
Calendar

Fechas límite flexibles

Restablece las fechas límite en función de tus horarios.
Clock

Sugerido: 6-8 hours/week

Aprox. 19 horas para completar
Comment Dots

English

Subtítulos: English

Programa - Qué aprenderás en este curso

1

Sección
Clock
4 horas para completar

Introduction

Description goes here...
Reading
5 videos (Total: 144 min), 6 readings, 1 quiz
Video5 videos
Lecture 2 - Biological Model Systems26m
Lecture 3 - Experimental Perturbations29m
Lecture 4 - Measuring Nucleic Acids29m
Lecture 5 - Measuring Protein and Protein States28m
Reading6 lecturas
Lecture Slides10m
Lecture Slides10m
Lecture Slide10m
Lecture Slides10m
Lecture Slides10m
Reading List10m
Quiz1 ejercicio de práctica
Quiz 140m

2

Sección
Clock
3 horas para completar

Deep mRNA Sequencing

Description goes here...
Reading
5 videos (Total: 93 min), 4 readings, 1 quiz
Video5 videos
Lecture 2 - 2nd and 3rd Generation Sequencing20m
Lecture 3 - Illumina-Based mRNA Sequencing19m
Lecture 4 - Lab-Based Video28m
Lecture 5 - mRNA Sequencing Data Analysis12m
Reading4 lecturas
Lecture Slides10m
Lecture Slides10m
Lecture Slides10m
Reading List10m
Quiz1 ejercicio de práctica
Quiz 220m

3

Sección
Clock
2 horas para completar

Mass Spectrometry-Based Proteomics

Description goes here...
Reading
6 videos (Total: 87 min), 3 readings, 1 quiz
Video6 videos
Lecture 2 - Basics of Mass Spectrometry 215m
Lecture 3 - Quantification in Proteomics15m
Lecture 4 - Lab-Based Video - Proteomics Day 117m
Lecture 5 - Lab-Based Video - Proteomics Day 216m
Lecture 6 - Lab-Based Video - Analysis7m
Reading3 lecturas
Lecture Slides10m
Lecture Slides10m
Reading List10m
Quiz1 ejercicio de práctica
Quiz 320m

4

Sección
Clock
1 hora para completar

Midterm Exam

Description goes here...
Reading
1 quiz
Quiz1 ejercicio de práctica
Midterm Exam34m
4.6
Briefcase

83%

consiguió un beneficio tangible en su carrera profesional gracias a este curso
Money

50%

consiguió un aumento de sueldo o ascenso

Principales revisiones

por FPJun 3rd 2016

Excellent course with a wealth of information about system biology technologies

por EMDec 4th 2015

Great course,\n\nRecommended if you are interest in new technologies

Instructor

Marc Birtwistle, PhD

Assistant Professor
Department of Pharmacology and Systems Therapeutics, Systems Biology Center New York (SBCNY)

Acerca de Icahn School of Medicine at Mount Sinai

The Icahn School of Medicine at Mount Sinai, in New York City is a leader in medical and scientific training and education, biomedical research and patient care....

Acerca del programa especializado Systems Biology and Biotechnology

Design systems-level experiments using appropriate cutting edge techniques, collect big data, and analyze and interpret small and big data sets quantitatively. The Systems Biology Specialization covers the concepts and methodologies used in systems-level analysis of biomedical systems. Successful participants will learn how to use experimental, computational and mathematical methods in systems biology and how to design practical systems-level frameworks to address questions in a variety of biomedical fields. In the final Capstone Project, students will apply the methods they learned in five courses of specialization to work on a research project....
Systems Biology and Biotechnology

Preguntas Frecuentes

  • Once you enroll for a Certificate, you’ll have access to all videos, quizzes, and programming assignments (if applicable). Peer review assignments can only be submitted and reviewed once your session has begun. If you choose to explore the course without purchasing, you may not be able to access certain assignments.

  • When you enroll in the course, you get access to all of the courses in the Specialization, and you earn a certificate when you complete the work. Your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.

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