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Experimental Methods in Systems Biology

Un vistazoProgramaPreguntas FrecuentesCreadoresTarifaCalificaciones y revisiones

InicioCiencias BiológicasBiología

Experimental Methods in Systems Biology

Escuela Icahn de Medicina del Monte Sinaí

Acerca de este curso: Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging. A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content.


Creada por:  Escuela Icahn de Medicina del Monte Sinaí
Escuela Icahn de Medicina del Monte Sinaí

  • Marc Birtwistle, PhD

    Enseñado por:  Marc Birtwistle, PhD, Assistant Professor

    Department of Pharmacology and Systems Therapeutics, Systems Biology Center New York (SBCNY)
Información básica
Curso 2 de 6 en Systems Biology and Biotechnology Specialization
Compromiso6-8 hours/week
Idioma
English
Cómo aprobarAprueba todas las tareas calificadas para completar el curso.
Calificaciones del usuario
4.6 estrellas
Calificación promedio del usuario 4.6Ve los que los estudiantes dijeron
Programa
SEMANA 1
Introduction
Description goes here
5 videos, 6 readings
  1. Vídeo: Lecture 1 -Scope and Overview
  2. Reading: Lecture Slides
  3. Vídeo: Lecture 2 - Biological Model Systems
  4. Reading: Lecture Slides
  5. Vídeo: Lecture 3 - Experimental Perturbations
  6. Reading: Lecture Slide
  7. Vídeo: Lecture 4 - Measuring Nucleic Acids
  8. Reading: Lecture Slides
  9. Vídeo: Lecture 5 - Measuring Protein and Protein States
  10. Reading: Lecture Slides
  11. Reading: Reading List
Calificado: Quiz 1
SEMANA 2
Deep mRNA Sequencing
Description goes here
5 videos, 4 readings
  1. Vídeo: Lecture 1 - History of Sequencing
  2. Vídeo: Lecture 2 - 2nd and 3rd Generation Sequencing
  3. Reading: Lecture Slides
  4. Vídeo: Lecture 3 - Illumina-Based mRNA Sequencing
  5. Reading: Lecture Slides
  6. Vídeo: Lecture 4 - Lab-Based Video
  7. Vídeo: Lecture 5 - mRNA Sequencing Data Analysis
  8. Reading: Lecture Slides
  9. Reading: Reading List
Calificado: Quiz 2
SEMANA 3
Mass Spectrometry-Based Proteomics
Description goes here
6 videos, 3 readings
  1. Vídeo: Lecture 1 - Basics of Mass Spectrometry 1
  2. Vídeo: Lecture 2 - Basics of Mass Spectrometry 2
  3. Reading: Lecture Slides
  4. Vídeo: Lecture 3 - Quantification in Proteomics
  5. Reading: Lecture Slides
  6. Vídeo: Lecture 4 - Lab-Based Video - Proteomics Day 1
  7. Vídeo: Lecture 5 - Lab-Based Video - Proteomics Day 2
  8. Vídeo: Lecture 6 - Lab-Based Video - Analysis
  9. Reading: Reading List
Calificado: Quiz 3
SEMANA 4
Midterm Exam
Description goes here
    Calificado: Midterm Exam
    SEMANA 5
    Flow and Mass Cytometry for Single Cell Protein Levels and Cell Fate
    Description goes here
    7 videos, 4 readings
    1. Vídeo: Lecture 1 - Flow Cytometry 1
    2. Vídeo: Lecture 2 - Flow Cytometry 2
    3. Reading: Lecture Slides
    4. Vídeo: Lecture 3 - Mass Cytometry
    5. Reading: Lecture Slides
    6. Vídeo: Lecture 4 - Cytometry Data Analysis
    7. Reading: Lecture Slides
    8. Vídeo: Lecture 5 - Lab-Based Video - Flow Cytometry - Acquisition
    9. Vídeo: Lecture 6 - Lab-Based Video - Flow Cytometry - Analysis
    10. Vídeo: Lecture 7 - Lab-Based Video - Mass Cytometry
    11. Reading: Reading List
    Calificado: Quiz 4
    SEMANA 6
    Live-cell Imaging for Single Cell Protein Dynamics
    Description goes here
    5 videos, 4 readings
    1. Vídeo: Lecture 1 - Fluorescence Microscopy
    2. Vídeo: Lecture 2 - Types of Imaging
    3. Reading: Lecture Slides
    4. Vídeo: Lecture 3 - Visualizing Molecules in Living Cells: Fluorescent Tools
    5. Reading: Lecture Slides
    6. Vídeo: Lecture 4 - Quantification
    7. Vídeo: Lecture 5 - Lab-Based Video
    8. Reading: Lecture Slides
    9. Reading: Reading List
    Calificado: Quiz 5
    SEMANA 7
    Integrating and Interpreting Datasets with Network Models and Dynamical Models
    Description goes here
    3 videos, 4 readings
    1. Vídeo: Lecture 1 - Omics data and Network Model Analyses
    2. Reading: Lecture Slides
    3. Vídeo: Lecture 2 - Single Cell Time Course Data and Dynamical Model Analyses
    4. Reading: Lecture Slides
    5. Vídeo: Lecture 3 - Dynamical Model Case Study
    6. Reading: Lecture Slides
    7. Reading: Reading List
    Calificado: Quiz 6
    SEMANA 8
    Final Exam
      Calificado: Final Exam

      Preguntas Frecuentes
      Cómo funciona
      Coursework
      Coursework

      Each course is like an interactive textbook, featuring pre-recorded videos, quizzes and projects.

      Help from Your Peers
      Help from Your Peers

      Connect with thousands of other learners and debate ideas, discuss course material, and get help mastering concepts.

      Certificates
      Certificates

      Earn official recognition for your work, and share your success with friends, colleagues, and employers.

      Creadores
      Escuela Icahn de Medicina del Monte Sinaí
      The Icahn School of Medicine at Mount Sinai, in New York City is a leader in medical and scientific training and education, biomedical research and patient care.
      Tarifa
      Comprar curso
      Accede a los materiales del curso

      Disponible

      Accede a los materiales con calificación

      Disponible

      Recibe una calificación final

      Disponible

      Obtén un Certificado de curso para compartir

      Disponible

      Calificaciones y revisiones
      Calificado 4.6 de 5 125 calificaciones

      MG

      it was so useful

      李俊宏

      The course is mainly focused on description of the technologies used in system biology. Mechanisms of these experiments may not be very clear which makes this course seems like an introduction to the experiments. Also the quizzes are too long with enormous words, sometimes it's like a game of finding differences. I hope the quizzes be more focused on principals and calculation, which will make it more clearer.

      Finally, the teacher and all staff have made a lot of efforts to build this nice course! Thank you so much!

      ML

      The course provides up to date understanding of new technologies. The examples are carefully analyzed and explained. The course is very helpful for those who want to follow lab-based research. However, learners must be aware that the amount of knowledge you take in for each lesson is very large, you might need to watch the lesson over and over again to actually understand deeply.

      JC

      Good lecture to know experimental method in lab



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