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Whole genome sequencing of bacterial genomes - tools and applications, Universidad Técnica de Dinamarca (DTU)

4.6
237 calificaciones
62 revisiones

Acerca de este Curso

This course will cover the topic of Whole genome sequencing (WGS) of bacterial genomes which is becoming more and more relevant for the medical sector. WGS technology and applications are high on international political agenda, as the classical methods are being replaced by WGS technology and therefore bioinformatic tools are extremely important for allowing the people working in this sector to be able to analyze the data and obtain results that can be interpreted and used for different purposes. The course will give the learners a basis to understand and be acquainted with WGS applications in surveillance of bacteria including species identification, typing and characterization of antimicrobial resistance and virulence traits as well as plasmid characterization. It will also give the opportunity to learners to learn about online tools and what they can be used for through demonstrations on how to use some of these tools and exercises to be solved by learners with use of freely available WGS analysis tools . By the end of this course you should be able to: 1. Describe the general Principles in typing of Bacteria 2. Give examples of the applications of Whole Genome Sequencing to Surveillance of bacterial pathogens and antimicrobial resistance 3. Apply genomic tools for sub-typing and surveillance 4. Define the concept of Next-Generation Sequencing and describe the sequencing data from NGS 5. Describe how to do de novo assembly from raw reads to contigs 6. Enumerate the methods behind the tools for species identification, MLST typing and resistance gene detection 7. Apply the tools for species identification, MLST typing and resistance gene detection in real cases of other bacterial and pathogen genomes. 8. Describe the methods behind the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing 9. Utilize the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing in real cases of other bacterial and pathogen genomes. 10. Explain the concept and be able to use the integrated bacterial analysis pipeline for batch analysis and typing of genomic data 11. Demonstrate how to construct phylogenetic tree based on SNPs 12. Apply the phylogenetic tool to construct phylogenetic trees and explain the relatedness of bacterial or pathogen strains 13. Describe how to create your own sequence database 14. Utilize the MyDbFinder tool to detect genetic markers of interest from whole genome sequencing...

Principales revisiones

por VL

Jan 14, 2018

This is a very interesting course, it's practical and simple to follow and I've learned a lot about whole genome sequencing and its techniques.

por EY

Oct 01, 2018

Good course for a beginner. Generally, help me understand whole genome sequencing of bacteria. Will follow other course that they have provide.

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62 revisiones

por Carol Hull-Jackson

Apr 01, 2019

The information presented was very good in terms of content. Sometimes the accent of the tutors was difficult to follow and the English translation of the text for the presentations were not always accurate.

por MUTSHIENE DEOGRATIAS EKWANZALA

Mar 29, 2019

Great and very insightful course. It really shed light on many aspects on whole genome sequencing

por Kumar virbhadra

Mar 17, 2019

Course contains relevant application based material. It was quite helpful.

por Matthew Young

Mar 03, 2019

Very much enjoyed the course and the practice problems involving Whole Genome Sequencing on the CGE website

por Adeyemi Samuel

Feb 20, 2019

Please, improvements on the bioinformatic tools and the zipped files will be appreciated, i had little difficulty opening and accessing them on my andriod device, however overall, the course was enlightening

por Robert Furrow

Jan 28, 2019

This course is mostly just an advertisement for the particular tools created by the CGE. It would have benefited from deeper explanations of the algorithms at many points. It didn't really build skills, though I am certainly more aware of the breadth of their online tools now.

por Lydia Mutanda

Jan 24, 2019

Such a good course which gives an overview to some of the bioinformatic tools that can be employed in genome sequencing. I'm glad I enrolled for this course because it has enlightened me of the other part of molecular biology I didn't know so much about.

So thanks to all the instructors that dedicated their time to provide us with new knowledge.

por Bikash Karki

Jan 15, 2019

Very simple and informative

por Ali Abdelkareem Ali Eisa

Jan 08, 2019

applications

por Sören Wacker

Jan 07, 2019

It is basically a commercial for their tools. Not much content to learn.