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Opiniones y comentarios de aprendices correspondientes a Whole genome sequencing of bacterial genomes - tools and applications por parte de Universidad Técnica de Dinamarca (DTU)

4.6
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1,060 calificaciones
283 reseña

Acerca del Curso

This course will cover the topic of Whole genome sequencing (WGS) of bacterial genomes which is becoming more and more relevant for the medical sector. WGS technology and applications are high on international political agenda, as the classical methods are being replaced by WGS technology and therefore bioinformatic tools are extremely important for allowing the people working in this sector to be able to analyze the data and obtain results that can be interpreted and used for different purposes. The course will give the learners a basis to understand and be acquainted with WGS applications in surveillance of bacteria including species identification, typing and characterization of antimicrobial resistance and virulence traits as well as plasmid characterization. It will also give the opportunity to learners to learn about online tools and what they can be used for through demonstrations on how to use some of these tools and exercises to be solved by learners with use of freely available WGS analysis tools . By the end of this course you should be able to: 1. Describe the general Principles in typing of Bacteria 2. Give examples of the applications of Whole Genome Sequencing to Surveillance of bacterial pathogens and antimicrobial resistance 3. Apply genomic tools for sub-typing and surveillance 4. Define the concept of Next-Generation Sequencing and describe the sequencing data from NGS 5. Describe how to do de novo assembly from raw reads to contigs 6. Enumerate the methods behind the tools for species identification, MLST typing and resistance gene detection 7. Apply the tools for species identification, MLST typing and resistance gene detection in real cases of other bacterial and pathogen genomes. 8. Describe the methods behind the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing 9. Utilize the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing in real cases of other bacterial and pathogen genomes. 10. Explain the concept and be able to use the integrated bacterial analysis pipeline for batch analysis and typing of genomic data 11. Demonstrate how to construct phylogenetic tree based on SNPs 12. Apply the phylogenetic tool to construct phylogenetic trees and explain the relatedness of bacterial or pathogen strains 13. Describe how to create your own sequence database 14. Utilize the MyDbFinder tool to detect genetic markers of interest from whole genome sequencing...

Principales reseñas

AP
19 de jul. de 2020

Thank you very much for this course through which i learnt basic handling of bioinformatics tools and concepts of WGS. This will help me a lot while pursuing my PhD research work in my field.

MB
9 de jun. de 2019

Excellent course with a wealth of information about tools and applications of whole genome sequencing. Presented in a very lucid and interesting manner, very crispy and simplified course.

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276 - 285 de 285 revisiones para Whole genome sequencing of bacterial genomes - tools and applications

por James P

12 de ago. de 2020

I thought this would be informative but the entire course is basically an infomercial for using their web based tools. Which to some, could be helpful but any serious bioinformatician should avoid.

por Luisa F D B

22 de jun. de 2020

I liked some of the sections but the course is really focused on the platform developed by its university, so despite it is useful it does not give examples of another tool like BLAST

por Victor B C B

5 de ago. de 2021

Poderiam explicar mais a fundo algumas definições e me pareceu que algumas ferramentas estão desatualizadas (Ex: Ferramenta para montagem, Velvet, a mais usada no momento é a Spades)

por Franziska N

1 de oct. de 2020

Good lectures, but unfortunately there is little general background transferred through this course. The lectures and the quiz seem mainly to be advertisement for their tools.

por Harvey T

29 de oct. de 2019

the presentation skills and the language were not so high. some files that need analysis are not working. But it is useful course by the end.Thx alot

por Jing C

14 de nov. de 2017

Wish it was more concise.

por Luka G

5 de jun. de 2020

The course itself is great, unfortunately the person who designed it and is the main speaker has incomprehensible English, to a point that one needs triple the exertion to understand her. The transcript of the speech seems to be automatically generated, so one can not even read the lecture to understand it.

por Catherine M

23 de oct. de 2020

Can not even pass the first quiz, even with correct answers. The two videos based off the questions don't answer some of the questions if at all, and a google search or more in depth search revealed the correct answers, however the quiz continued to be incorrect no matter what correct answers you gave it.

por Piotr

29 de jun. de 2021

W​eek 1 videos just throw a bunch of acronyms, without explaining anything, not even in depth, but in their relevance for the topic of the course. Similiarly, later weeks continously bring more terms with little explanation of them and providing little logic how are they connected to each other. Quizes don't relate to informations from vidoes anyhow or do so only in some of questions. Quizes don't provide any feedback.

por Gail C

18 de oct. de 2021

T​he assignments are very difficult and I dont think the lectures provide enough information to do the assignments. Dont recommend this course.